COLLECTING / SUBMITTING

D N A

 

When registration opens you will need a form from MBOAR to submit samples.

The form will be available after January 28th 2021.

 

The owner of the animal will need the sample number/sample ID, when filling in the form, to register a breeder pig. The DNA can be used in the future to verify a pig or for testing of parenting matches when needed.

 

What is the best way to collect a good DNA sample?

Tissue Samples: MBOAR advises its members to order and use the tissue sample gun / Tissue Sampling (TSU) Applicator, to collect DNA from both a piglet and an adult pig. The TSU may be purchased at most retailers offering veterinary supplies, however below is a link to purchase one of it directly from GeneSeek.

Ear Tagging and collecting a sample at the same time:

The TSU is an easy tool to use, that collects a tissue sample from the ear of the pig. Link: Tissue Sampling (TSU) Applicator,

It can also be used to ear tag your pigs and collect a sample in one step! It is however necessary that you order the more expensive 840 HDX EID ear tags, in order to accomplish this task.

Link: Tissue Sampling Units w/ 840 USDA HDX EID Ear Tags

 

This technique rarely fails to extract the DNA necessary for GeneSeek to successfully process the sample. Often samples fail when hair is pulled and submitted because of dirt on the hair or lack of sufficient follicles in a sample.

 

Here a link to the Australian website from GeneSeek where you will find a good explanation about the reasons a hair, blood or tissue sample might fail. 

Link: COMMON REASONS FOR SAMPLE FAILURES

 

Important note:

It's NOT possible to get good follicles (hair roots) from a Mangalitsa piglet under 5 - 7 months of age!!

Hair samples & blood samples:

Other  possibilities to collect DNA, is by hair or blood samples. Both methods are explained in detailed below. Collecting blood from a piglet is not an easy task!

 

MBOAR promotes the most animal friendly DNA collecting:

Collecting a tissue sample with the TSU!

 

Word from GeneSeek:

Recording a sample

How and when to collect DNA samples to produce the best genomic test results?

"Whether the sample is from tissue or another source, recording it involves associating an identification number for the animal with the identification number of the sample, and is arguably the most important part of the process — though seldom recognized as such.

More than half of the focus for development of efficient sample collection systems is around the process of associating the sample barcode with the animal’s permanent I.D. to ensure “chain of custody” — like a crime scene investigation.

The sample barcode is the vital piece of information from the lab perspective, but in the end, the information must be related to the animal I.D. to be useful for the dairyman. Sample identification and animal I.D. recording can be done by hand, however, this method can be time-consuming and lead to transcription errors."

 

You can purchase the:

Allflex TSU Applicator (Part# 528)

Allflex TSUs (Pack of 10) (Tubes for the TSU) (Part #531)

Blood Cards (Part #525) or

 Hair Card (Part #527)

All items you will find at the GeneSeek order-website:

More information on how to take samples can be found here: Click on the box and a PDF/VIDEO will open with more information

TSU / Tissue sample gun

Hair card

 

Blood card

Detailed guidelines how to pull hair and add them to the cards,

you can find here:

- GUIDELINES -

 

We advise pulling the longer hair from the neck of a Mangalitsa pig with a pair of pliers.  It is vital that you do not use any tool that will cut the hair as you need the follicles!

 

 

 

 UNDER 

 CONSTRUCTION

 

For the next few weeks MBOAR welcomes everyone to become a member.

 

Some details on DNA testing need to be worked out with GeneSeek in Nebraska. As soon as this is finalised, (January 28th 2021) MBOAR will open up the Register/Herd-book for Mangalitsa pigs with verified lineage.

After you have became an MBOAR Member, you can start completing

STEP 1 & STEP 2 which are required to register stock. We advise to wait with collecting DNA from the pigs you would like to get registered at MBOAR, till the Register/Herd-book is officially is open and we can provide you with all needed information.

Meanwhile:

1. Get your Premise ID / Farm PIN

2. Order 840 ear tags / tag your breeding stock

 

3. Order DNA testing supplies from NEOGEN (formerly GeneSeek)

4. DNA / collect DNA from each pig you would like to register (ON HOLD!)

More about these steps you can find when you go to BREED REGISTRY (main menu)

To register your Mangalitsa pig in the MBOAR Register, a DNA sample of the pig/piglet is required.

 

Please make sure you include all the information according to these guidelines as it is essential for the accuracy of the Register!!

TUS - SAMPLING UNIT/GUN:

The TSU - Tissue Sampling Unit/Gun and the Sampling Unit that you can order from GeneSeek/Neogen. 

TSU - Tissue Sampling Unit Applicator_ G
TSU - DNA Tissue Sampling Unit _ GeneSee
Tissue Sample TUS_GeneSeek_MBOAR Mangali

This picture shows you the different parts of the Tissue Sample Unit/Tube.

 

In the 3 videos below you can watch how to use this equipment and what is important to know when usingthe TSU Gun to collect DNA from your animals.

Please watch them carefully, as this process is essential to register an animal in MBOAR. One of the most important steps is that your sample is correctly 'sealed' in the tube (in Video 2) and that the 840 Tag number/ear tag number of the animal is connected to the code of the tube (in Video 2)!

If numbers are not correctly match parenting testing will fail fail when it matters the most. So taking samples and connecting them to the individual pigs ID (ear tag/notch) is one of THE most important parts of the register. 

HAIR / BLOOD CARDS:

Below you can see how to fill in the Hair or Blood Cards:

You need to add your MBOAR Member ID to the sample. To avoid confusion, please draw a line through the number zero = Ø so we do not confuse it with the letter O. 

GeneSeek Hair Card 1- MBOAR ID_text.jpg
GeneSeek Hair Card 2_ MBOAR text jpg.jpg
Hair follicles : roots  GeneSeek _MBOAR.
MBOAR_Mangalitsa _ Hair card GeneSeek