Please complete step 1-3, we ask you to read especially step 4 carefully and follow the guidelines!
S T E P 4:
COLLECTING / SUBMITTING
D N A
For future parenting testing from your offspring!
After you have became an MBOAR Member, you need to have the following steps completed:
1. Get your Premise ID / Farm PIN
The USDA Farm Premise Identification Number (PIN) is a unique identifier that is required to maintain a traceability record of livestock that crosses state lines, enters a USDA slaughterhouse, or to alert and contact when and if there is a health and safety emergency.
Your USDA Farm (PIN) is unique to the address where you raise your animals and is required for your farm ear tag system. This identification will make it possible to keep track of the pure Mangalitsas in the USA in the future.
More information on where to order your Farm (PIN): https://www.aphis.usda.gov/aphis/ourfocus/animalhealth/traceability/state-pin/state-pin
2. Order 840 ear tags / tag your breeding stock
Once you have your USDA Farm (PIN) assigned, you should obtain USDA 840 ear tags for swine (button sized seems best) for each of your registered animals and for any new piglets you intend to register.
There is a great selection of tags and applicators at Valley Vet. We don't recommend a specific vendor over another. However, they offer an extensive inventory of tags and applicators for any type of livestock.
An example of a small USDA 840 tag and a possible second additional bigger ear tag, that can be used as your farm tag you can be seen here: Picture of 2 tag system
For the ones who are new to pigs, here a good video how and where to tag a pig:
3. DNA testing supplies from NEOGEN & submitting the samples
To register a Mangalitsa in the MBOAR herd-book, DNA (Hair, Tissue or Blood) must be gathered from the breeding animals you want to register. The sample number, on the card/tube, must be submitted during the online registration!
After you have filled in the online form with all required data and payed the fee, MBOAR will send you an email. You have to print out the file attached to the email and send it to NeoGen with your samples.
NeoGen needs this MBOAR document to process the DNA.
Please do not send any DNA to NeoGen without the MBOAR form!!
Watch this video to understand the process correctly: MBOAR Animal Registration
The DNA data will remain on file and be used to show proof of parentage for future piglets by DNA. Collecting DNA can be done by taking a blood sample, hair sample or tissue sample. Blood and hair sample cards can be ordered from NEOGEN.
All these items you will find at the GeneSeek/NEOGEN order-website:
Allflex TSUs (Pack of 10) (Tubes for the TSU) (Part #531),
4. DNA /collect DNA from each pig you would like to register
Here a guideline how to pull hair, take blood or tissue samples:
We advise pulling the longer hair from the neck of a Mangalitsa pig with a pair of pliers. It is vital that you do not use any tool that will cut the hair as you need the follicles!
MBOAR promotes tissue sampling (with TSU Gun) as it is the most animal friendly method for the piglets and the most reliable method to collect DNA. Anyhow taking a tissue-sample for a full gown pig is not easy, so pulling hair from older pigs (12 plus moth) might be an easier way.
NEOGEN (GeneSeek) told us it's not easy to collect a good hair sample from a pig younger than 7-8 month of age as the follicles are often staying in the hide of the young pig! So MBOAR promptes to use the TSU Gun to get DNA samples from piglets and stock till 12 month of age.
If you do not want to use the Tissue sample method Karla Denton (an MBOAR member) made this video to show how to pull hair from a piglet:
In the videos below you can watch how to use this equipment and what is important to know when using the TSU Gun to collect DNA from your animals.
Please watch them carefully, as this process is essential to register an animal in MBOAR. One of the most important steps is that your sample is correctly 'sealed' in the tube (in Video 2) and that the 840 Tag number/ear tag number of the animal is connected to the code of the tube (in Video 2)!
If numbers are not correctly match parenting testing will fail fail when it matters the most. So taking samples and connecting them to the individual pigs ID (ear tag/notch) is one of THE most important parts of the register
The pictures above shows you the different parts of the Tissue Sample Unit/Tube. How to fill in the Hair or Blood cards you order from Neogen/GeneSeek and the hair that needs to go on the Hair card.
Here a link to the Australian website from GeneSeek where you will find a good explanation about the reasons a hair, blood or tissue sample might fail.
Word from NEOGEN / GeneSeek:
How and when to collect DNA samples to produce the best genomic test results?
"Whether the sample is from tissue or another source, recording it involves associating an identification number for the animal with the identification number of the sample, and is arguably the most important part of the process — though seldom recognized as such.
More than half of the focus for development of efficient sample collection systems is around the process of associating the sample barcode with the animal’s permanent I.D. to ensure “chain of custody” — like a crime scene investigation.
The sample barcode is the vital piece of information from the lab perspective, but in the end, the information must be related to the animal I.D. to be useful for the dairyman. Sample identification and animal I.D. recording can be done by hand, however, this method can be time-consuming and lead to transcription errors."
- ANIMAL REGISTRATION -
When you have lineage (minimum 3 generations or proof that your pig is offspring of the imports) and you have completed step 1-4 you are ready to register your stock.
We have produced a short video of the DNA submission process via MBOAR. If you have questions on any of the 4 steps at the moment please contact MBOAR.
The video you can find here: ANIMAL REGISTRATION
Click on the button and you will be taken to the Registration-website (www.mboar.org) where you will find more about the registration process and the MBOAR Animal Registration Forms.